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normal cell lines hmec  (ATCC)


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    ATCC normal cell lines hmec
    Normal Cell Lines Hmec, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1326 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+normal+mammary+epithelial+cells/Primary+Mammary+Epithelial+Cells%3B+Normal%2C+Human/pm42263414-39-14-25
    Average 99 stars, based on 1326 article reviews
    normal cell lines hmec - by Bioz Stars, 2026-09
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    Multiple Displacement Amplification:

    Article Title: ZWINT down-regulated by miR-495-3p inhibited lung metastasis of breast cancer by blocking p38 MAPK signaling pathway activation.
    Article Snippet: .. Human normal mammary epithelial cells (MCF-10A) and breast tumor cell lines (MCF-7 and MDA-MB-231) were obtained from the American Type Culture Collection (ATCC). .. These cells were cultured in RPMI-1640 medium (Pricellla, Wuhan, China) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin.

    Article Title: Cuproptosis- and m6A-related lncRNA prognostic signature for breast cancer, in which Z68871.1 contributes to triple-negative breast cancer progression.
    Article Snippet: Breast cancer (BC) has the second-highest global incidence rate among all cancers and poses a great threat to human health.. Cuproptosis, a newly discovered type of cell death, is expected to become a promising target for cancer therapy.. Moreover, N6-methyladenosine (m6A) modification has been shown to be involved in a variety of cell death pathways that affect malignant tumor development; however, the relationship between cuproptosis and m6A modification remains unclear.

    Article Title: ZWINT down-regulated by miR-495-3p inhibited lung metastasis of breast cancer by blocking p38 MAPK signaling pathway activation
    Article Snippet: .. Human normal mammary epithelial cells (MCF-10A) and breast tumor cell lines (MCF-7 and MDA-MB-231) were obtained from the American Type Culture Collection (ATCC). .. These cells were cultured in RPMI-1640 medium (Pricellla, Wuhan, China) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin.

    Article Title: lncRNA SEMA3B-AS1 Inhibits miR-513c-5p to Regulate the Progression of Triple-negative Breast Cancer.
    Article Snippet: Background/Aim: Triple-negative breast cancer (TNBC) is the most malignant breast cancer subtype with a short survival time and high morality.. There is an urgent need for effective indicators able to predict tumor progression and provide reference for adjusting the therapeutic strategy of TNBC. lncRNA semaphorin 3B antisense RNA1-AS1 (SEMA3B-AS1) was previously identified to be correlated with the stemness and autophagy of breast cancer.. SEMA3B-AS1’s role in TNBC was investigated in the present study, aiming to explore a novel biomarker for the development and prognosis of TNBC.

    Article Title: Identification of mitochondrial permeability transition-related lncRNAs as quantitative biomarkers for the prognosis and therapy of breast cancer
    Article Snippet: .. Human normal mammary epithelial cells (MCF-10A) and breast cancer cells (SK-BR-3, MCF-7, HCC1806, BT549, MDA-MB-231, and Taxol-resistant MDA-MB-231) were purchased from the American Type Culture Collection (ATCC). .. The abovementioned cells were cultured in DMEM or RPMI 1640 medium (Gibco, CA, United States) supplemented with 10% FBS in a standard humidified incubator at 37°C with 5% CO 2 .

    Cell Culture:

    Article Title: Lycorine weakens tamoxifen resistance of breast cancer via abrogating HAGLR-mediated epigenetic suppression on VGLL4 by DNMT1.
    Article Snippet: .. Human normal mammary epithelial cells (MCF-10A cells) and the TAMsensitive human BC cell lines (MCF7 and T47D cells) were purchased from American type culture collection (ATCC) and cultured in DMEM (Gibco) containing 10% FBS (Gibco) at 37 C with 5% CO2. .. TAMR BC cells were constructed as previously described.20 In brief, MCF7 and T47D cells were treated with 1 μM 4-hydroxytamoxifen (Sigma-Aldrich) for 3 weeks and then 100 nM 4-hydroxytamoxifen for 6 months.

    Clone Assay:

    Article Title: Screening and Mechanistic Investigation of Food-Derived Multifunctional ACE-Inhibitory Tri-Peptides
    Article Snippet: .. Human embryonic kidney cells (HEK-293T), mouse embryonic fibroblast cells (NIH-3T3), human normal mammary epithelial cells (MCF-10A), and human cloned colon adenocarcinoma cells (Caco-2) were all acquired from the American Type Culture Collection. ..



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    PCMT1 is involved in PTX resistance of BC cells, a process potentially involving COX-2-mediated AA metabolism. (A) RT-qPCR analysis of PCMT1 mRNA levels in tumor tissues and paired adjacent normal tissues from 30 BC patients treated with PTX. (B) Immunoblotting analysis of PCMT1 and COX-2 protein levels in tumor tissues and paired adjacent normal tissues from PTX-treated BC patients (n = 10; 5 PTX-sensitive and 5 PTX-resistant). (C) Correlation between PCMT1 and COX-2 expression was assessed using the Pearson correlation coefficient. (D) RT-qPCR analysis of PCMT1 mRNA levels in <t>MCF10A,</t> BC cells (MDA-MB-231, MCF-7), and PTX-resistant cells (MDA-MB-231/PTX, MCF-7/PTX) (n = 3). (E) Immunoblotting analysis of PCMT1 and COX-2 protein levels in BC cells and PTX-resistant cells (n = 3). (F) IC50 values of BC cells and PTX-resistant cells assessed using the CCK-8 assay (n = 3). Values are represented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.
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    PCMT1 is involved in PTX resistance of BC cells, a process potentially involving COX-2-mediated AA metabolism. (A) RT-qPCR analysis of PCMT1 mRNA levels in tumor tissues and paired adjacent normal tissues from 30 BC patients treated with PTX. (B) Immunoblotting analysis of PCMT1 and COX-2 protein levels in tumor tissues and paired adjacent normal tissues from PTX-treated BC patients (n = 10; 5 PTX-sensitive and 5 PTX-resistant). (C) Correlation between PCMT1 and COX-2 expression was assessed using the Pearson correlation coefficient. (D) RT-qPCR analysis of PCMT1 mRNA levels in MCF10A, BC cells (MDA-MB-231, MCF-7), and PTX-resistant cells (MDA-MB-231/PTX, MCF-7/PTX) (n = 3). (E) Immunoblotting analysis of PCMT1 and COX-2 protein levels in BC cells and PTX-resistant cells (n = 3). (F) IC50 values of BC cells and PTX-resistant cells assessed using the CCK-8 assay (n = 3). Values are represented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Frontiers in Pharmacology

    Article Title: LITAF suppresses breast cancer and paclitaxel resistance by ubiquitinating and degrading PCMT1 to inhibit COX-2-dependent arachidonic acid metabolism

    doi: 10.3389/fphar.2026.1706420

    Figure Lengend Snippet: PCMT1 is involved in PTX resistance of BC cells, a process potentially involving COX-2-mediated AA metabolism. (A) RT-qPCR analysis of PCMT1 mRNA levels in tumor tissues and paired adjacent normal tissues from 30 BC patients treated with PTX. (B) Immunoblotting analysis of PCMT1 and COX-2 protein levels in tumor tissues and paired adjacent normal tissues from PTX-treated BC patients (n = 10; 5 PTX-sensitive and 5 PTX-resistant). (C) Correlation between PCMT1 and COX-2 expression was assessed using the Pearson correlation coefficient. (D) RT-qPCR analysis of PCMT1 mRNA levels in MCF10A, BC cells (MDA-MB-231, MCF-7), and PTX-resistant cells (MDA-MB-231/PTX, MCF-7/PTX) (n = 3). (E) Immunoblotting analysis of PCMT1 and COX-2 protein levels in BC cells and PTX-resistant cells (n = 3). (F) IC50 values of BC cells and PTX-resistant cells assessed using the CCK-8 assay (n = 3). Values are represented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Human normal mammary epithelial cells MCF10A (ATCC, USA) were cultured in DMEM/F12 (Procell) supplemented with HS (5%), EGF (20 ng/mL), hydrocortisone (0.5 μg/mL), insulin (0.5 μg/mL), NEAA (1%), and P/S (1%).

    Techniques: Quantitative RT-PCR, Western Blot, Expressing, CCK-8 Assay